Project

Part:BBa_K329019

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-24)


Population counter Tetracycline resistant - Final PmS-MTet

This part is our "final" population counter with Tetracycline resistance, RFP fluorescence and timer device. However the cells express constitutively RFP without reasonable explanation...

When IntI1 (BBa_K329014) is expressed, it excises the double terminator flanked by attC sites (BBa_J99001) and triggers the expression of TetA(C) (with a medium RBS, BBa_K329000), mRFP1 and LuxI (BBa_K081008) because of the constitutive promoter upstream. LuxI produces HSL which binds to LuxR (expressed constitutively), then activates the transcription of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1294
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3005
    Illegal BamHI site found at 1440
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1466
    Illegal NgoMIV site found at 1834
    Illegal NgoMIV site found at 1994
    Illegal AgeI site found at 3592
    Illegal AgeI site found at 3704
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3886
    Illegal BsaI.rc site found at 4614


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